YNB+Nitrogen-FeCl3 Powder, 50 grams

Catalog #: 1551-050

Recipe in mg/L:

Salts, Vitamins, Minerals & Trace Elements

  • Biotin (0.002 mg/L)
  • Boric acid (0.5 mg/L)
  • Calcium chloride dihydrate (100 mg/L)
  • Copper (II) sulfate pentahydrate (0.04 mg/L)
  • Folic acid (0.002 mg/L)
  • Inositol (In Base Formula) (2 mg/L)
  • Magnesium sulfate anhydrous (500 mg/L)
  • Manganese sulfate monohydrate (0.4 mg/L)
  • Niacin (0.4 mg/L)
  • 4-Aminobenzoic acid (PABA) (In Base Formula) (0.2 mg/L)
  • D-Pantothenic acid hemicalcium salt (0.4 mg/L)
  • Potassium iodide (0.1 mg/L)
  • Potassium phosphate monobasic anhydrous (1000 mg/L)
  • Pyridoxine hydrochloride (0.4 mg/L)
  • Riboflavin (0.2 mg/L)
  • Sodium chloride (100 mg/L)
  • Sodium molybdate (0.2 mg/L)
  • Thiamine hydrochloride (0.4 mg/L)
  • Zinc sulfate monohydrate (0.4 mg/L)

Nitrogen

  • Ammonium sulfate (5000 mg/L)

Instructions

Commonly added at the suggested g/L to a sugar and an amino acid mixture. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes. NOTE: While FeCl3 has not been added to this product, trace amounts of iron may be inadvertently introduced into the media from water, glassware, or other sources.  If complete elimination of iron is necessary for downstream applications, removal using chelation or other methods may be necessary.

  • Suggested g/L: 6.71
  • Storage Temperature: Room Temp

$7700