SC-Met Powder, 30 grams
Catalog #: 1343-030Recipe in mg/L:
Amino Acids & Supplements
- Adenine hemisulfate (21 mg/L)
- L-Alanine (85.6 mg/L)
- L-Arginine (85.6 mg/L)
- L-Asparagine monohydrate (85.6 mg/L)
- L-Aspartic acid (85.6 mg/L)
- L-Cysteine hydrochloride monohydrate (85.6 mg/L)
- L-Glutamic acid (85.6 mg/L)
- L-Glutamine (85.6 mg/L)
- L-Glycine (85.6 mg/L)
- L-Histidine hydrochloride monohydrate (85.6 mg/L)
- DL-Homoserine (85.6 mg/L)
- Inositol (in AA/Supplement Mix) (85.6 mg/L)
- L-Isoleucine (85.6 mg/L)
- L-Leucine (173.4 mg/L)
- L-Lysine hydrochloride (85.6 mg/L)
- 4-Aminobenzoic acid (PABA) (In AA/Supplement Mix) (8.6 mg/L)
- L-Phenylalanine (85.6 mg/L)
- L-Proline (85.6 mg/L)
- L-Serine (85.6 mg/L)
- L-Tryptophan (85.6 mg/L)
- L-Tyrosine (85.6 mg/L)
- L-Valine (85.6 mg/L)
- Uracil (85.6 mg/L)
Instructions
Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes. To prepare plates, autoclave agar separately and add to sterile medium, or add agar to liquid medium and adjust to pH 5.8-6.0 before autoclaving. Note: Threonine has been replaced by homoserine due to their relationship to methionine biosynthesis.
- Suggested g/L: 1.92
- Storage Temperature: 2-8 C