For a 1X solution, add pouch contents to 1000mL deionized water. For a 2X solution, add pouch contents to 500mL deionized water. Adjust pH to 4.75 with 6N NaOH and filter sterilize. NOTE: This optimized formulation contains several highly-hydrated ingredients. Some clumping of powder may be expected and should be easily dispersed when stirred to dissolve. Store powder and prepared medium at 2C-8C.