DOB (Dropout Base) Powder, 1 kg
Catalog #: 1650-1KGRecipe in mg/L:
Salts, Vitamins, Minerals & Trace Elements
- Biotin (0.002 mg/L)
- Boric acid (0.5 mg/L)
- Calcium chloride dihydrate (100 mg/L)
- Copper (II) sulfate pentahydrate (0.04 mg/L)
- Folic acid (0.002 mg/L)
- Inositol (In Base Formula) (2 mg/L)
- Iron (III) chloride (0.2 mg/L)
- Magnesium sulfate anhydrous (500 mg/L)
- Manganese sulfate monohydrate (0.4 mg/L)
- Niacin (0.4 mg/L)
- 4-Aminobenzoic acid (PABA) (In Base Formula) (0.2 mg/L)
- D-Pantothenic acid hemicalcium salt (0.4 mg/L)
- Potassium iodide (0.1 mg/L)
- Potassium phosphate monobasic anhydrous (1000 mg/L)
- Pyridoxine hydrochloride (0.4 mg/L)
- Riboflavin (0.2 mg/L)
- Sodium chloride (100 mg/L)
- Sodium molybdate (0.2 mg/L)
- Thiamine hydrochloride (0.4 mg/L)
- Zinc sulfate monohydrate (0.4 mg/L)
Nitrogen
- Ammonium sulfate (5000 mg/L)
Sugar
- Glucose (20000 mg/L)
Instructions
Use at suggested g/L and supplement with an amino acid mixture (CSM, SC, HSM or BSM) if desired. Mix with stirring for 10-15 minutes and filter sterilize or autoclave at 121°C for 15 minutes. Note: If using an SC amino acid mixture to prepare plates, autoclave agar separately and add to sterile medium, or add agar to liquid medium and adjust to pH 5.8-6.0 before autoclaving.
- Suggested g/L: 26.71
- Storage Temperature: Room Temp