PMG (Pombe Minimal Glutamate) Powder, 500 grams
Catalog #: 2060-500Recipe in mg/L:
Salts, Vitamins, Minerals & Trace Elements
- Biotin (0.01 mg/L)
- Boric acid (0.5 mg/L)
- Calcium chloride dihydrate (14.7 mg/L)
- Citric acid trisodium dihydrate (1 mg/L)
- Copper (II) sulfate pentahydrate (0.04 mg/L)
- Inositol (In Base Formula) (10 mg/L)
- Iron (III) chloride (0.138 mg/L)
- Magnesium chloride anhydrous (492 mg/L)
- Manganese sulfate monohydrate (0.4 mg/L)
- Niacin (10 mg/L)
- D-Pantothenic acid hemicalcium salt (1 mg/L)
- Potassium chloride (1000 mg/L)
- Potassium iodide (0.1 mg/L)
- Potassium phthalate monobasic (3000 mg/L)
- Sodium molybdate (0.04 mg/L)
- Sodium phosphate dibasic anhydrous (2200 mg/L)
- Sodium sulfate anhydrous (40 mg/L)
- Zinc sulfate monohydrate (0.4 mg/L)
Amino Acids & Supplements
- L-Glutamic acid (3750 mg/L)
Sugar
- Glucose (20000 mg/L)
Instructions
Add at suggested g/L to diH2O. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes. To prepare plates, autoclave agar separately and add to sterile medium, or add agar to liquid medium and adjust to pH 5.8-6.0 before autoclaving.
- Suggested g/L: 30.52
- Storage Temperature: Room Temp