YNB+Nitrogen-CuSO4-FeCl3 Powder, 250 grams
Catalog #: 1527-250Recipe in mg/L:
Salts, Vitamins, Minerals & Trace Elements
- Biotin (0.002 mg/L)
- Boric acid (0.5 mg/L)
- Calcium chloride dihydrate (100 mg/L)
- Folic acid (0.002 mg/L)
- Inositol (In Base Formula) (2 mg/L)
- Magnesium sulfate anhydrous (500 mg/L)
- Manganese sulfate monohydrate (0.4 mg/L)
- Niacin (0.4 mg/L)
- 4-Aminobenzoic acid (PABA) (In Base Formula) (0.2 mg/L)
- D-Pantothenic acid hemicalcium salt (0.4 mg/L)
- Potassium iodide (0.1 mg/L)
- Potassium phosphate monobasic anhydrous (1000 mg/L)
- Pyridoxine hydrochloride (0.4 mg/L)
- Riboflavin (0.2 mg/L)
- Sodium chloride (100 mg/L)
- Sodium molybdate (0.2 mg/L)
- Thiamine hydrochloride (0.4 mg/L)
- Zinc sulfate monohydrate (0.4 mg/L)
Nitrogen
- Ammonium sulfate (5000 mg/L)
Instructions
Commonly added at the suggested g/L to a sugar and an amino acid mixture. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes. While CuSO4 and FeCl3 have not been added to this product, trace amounts of these ingredients may be inadvertently introduced into the media from water, glassware, or other sources. If complete elimination is required for downstream applications, removal using chelation or other methods may be necessary.
- Suggested g/L: 6.71
- Storage Temperature: Room Temp
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