2X ACCM-D (-Glucose-Lys) Powder, 10 x 0.5 liter pouches

Catalog #: 4705-300

Recipe in mg/L:

Salts, Vitamins, Minerals & Trace Elements

  • Calcium chloride dihydrate (26.4 mg/L)
  • Citric acid (5136 mg/L)
  • Citric acid trisodium dihydrate (9480 mg/L)
  • Iron (II) sulfate heptahydrate (5.6 mg/L)
  • Magnesium chloride hexahydrate (400 mg/L)
  • Potassium phosphate monobasic anhydrous (1000 mg/L)
  • Sodium chloride (14560 mg/L)

Amino Acids & Supplements

  • L-Alanine (224 mg/L)
  • L-Arginine (316 mg/L)
  • L-Asparagine monohydrate (176 mg/L)
  • L-Aspartic acid (144 mg/L)
  • L-Cysteine hydrochloride monohydrate (548 mg/L)
  • L-Glutamic acid potassium salt monohydrate (1344 mg/L)
  • L-Glutamine (712 mg/L)
  • L-Glycine (176 mg/L)
  • L-Histidine hydrochloride monohydrate (110 mg/L)
  • L-Isoleucine (224 mg/L)
  • L-Leucine (462 mg/L)
  • L-Methionine (136 mg/L)
  • L-Phenylalanine (208 mg/L)
  • L-Proline (696 mg/L)
  • L-Serine (354 mg/L)
  • L-Threonine (244 mg/L)
  • L-Tryptophan (100 mg/L)
  • L-Tyrosine (218 mg/L)
  • L-Valine (322 mg/L)

Other Additives

  • Methyl-b-cyclodextrin (2000 mg/L)
  • RPMI 1640-Glutamine-Amino acids-Glucose (2000 mg/L)

Instructions

For a 1X solution, add pouch contents to 1000mL deionized water. For a 2X solution, add pouch contents to 500mL deionized water. Adjust pH to 4.75 with 6N NaOH and filter sterilize. NOTE: This optimized formulation contains several highly-hydrated ingredients. Some clumping of powder may be expected and should be easily dispersed when stirred to dissolve. Store powder and prepared medium at 2C-8C.

  • Suggested g/L: 41.32
  • Storage Temperature: 2-8 C

$89500