DOB-Glucose w/ 2% Raffinose Powder, 250 grams

Catalog #: 1654-250

Recipe in mg/L:

Salts, Vitamins, Minerals & Trace Elements

  • Biotin (0.002 mg/L)
  • Boric acid (0.5 mg/L)
  • Calcium chloride dihydrate (100 mg/L)
  • Copper (II) sulfate pentahydrate (0.04 mg/L)
  • Folic acid (0.002 mg/L)
  • Inositol (In Base Formula) (2 mg/L)
  • Iron (III) chloride (0.2 mg/L)
  • Magnesium sulfate anhydrous (500 mg/L)
  • Manganese sulfate monohydrate (0.4 mg/L)
  • Niacin (0.4 mg/L)
  • 4-Aminobenzoic acid (PABA) (In Base Formula) (0.2 mg/L)
  • D-Pantothenic acid hemicalcium salt (0.4 mg/L)
  • Potassium iodide (0.1 mg/L)
  • Potassium phosphate monobasic anhydrous (1000 mg/L)
  • Pyridoxine hydrochloride (0.4 mg/L)
  • Riboflavin (0.2 mg/L)
  • Sodium chloride (100 mg/L)
  • Sodium molybdate (0.2 mg/L)
  • Thiamine hydrochloride (0.4 mg/L)
  • Zinc sulfate monohydrate (0.4 mg/L)

Nitrogen

  • Ammonium sulfate (5000 mg/L)

Sugar

  • Raffinose pentahydrate (20000 mg/L)

Instructions

Use at suggested g/L and supplement with an amino acid mixture (CSM, SC, HSM or BSM) if desired. Mix with stirring for 10-15 minutes and filter sterilize or autoclave at 121°C for 15 minutes. Note: If using an SC amino acid mixture to prepare plates, autoclave agar separately and add to sterile medium, or add agar to liquid medium and adjust to pH 5.8-6.0 before autoclaving.

  • Suggested g/L: 26.71
  • Storage Temperature: Room Temp

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