2X ACCM-D (-Glucose-Arg-Lys) Powder, 10 x 0.5 liter pouches
Catalog #: 4704-300Recipe in mg/L:
Salts, Vitamins, Minerals & Trace Elements
- Calcium chloride dihydrate (26.4 mg/L)
- Citric acid (5136 mg/L)
- Citric acid trisodium dihydrate (9480 mg/L)
- Iron (II) sulfate heptahydrate (5.6 mg/L)
- Magnesium chloride hexahydrate (400 mg/L)
- Potassium phosphate monobasic anhydrous (1000 mg/L)
- Sodium chloride (14560 mg/L)
Amino Acids & Supplements
- L-Alanine (224 mg/L)
- L-Asparagine monohydrate (176 mg/L)
- L-Aspartic acid (144 mg/L)
- L-Cysteine hydrochloride monohydrate (548 mg/L)
- L-Glutamic acid potassium salt monohydrate (1344 mg/L)
- L-Glutamine (712 mg/L)
- L-Glycine (176 mg/L)
- L-Histidine hydrochloride monohydrate (110 mg/L)
- L-Isoleucine (224 mg/L)
- L-Leucine (462 mg/L)
- L-Methionine (136 mg/L)
- L-Phenylalanine (208 mg/L)
- L-Proline (696 mg/L)
- L-Serine (354 mg/L)
- L-Threonine (244 mg/L)
- L-Tryptophan (100 mg/L)
- L-Tyrosine (218 mg/L)
- L-Valine (322 mg/L)
Other Additives
- Methyl-b-cyclodextrin (2000 mg/L)
- RPMI 1640-Glutamine-Amino acids-Glucose (2000 mg/L)
Instructions
For a 1X solution, add pouch contents to 1000mL deionized water. For a 2X solution, add pouch contents to 500mL deionized water. Adjust pH to 4.75 with 6N NaOH and filter sterilize. NOTE: This optimized formulation contains several highly-hydrated ingredients. Some clumping of powder may be expected and should be easily dispersed when stirred to dissolve. Store powder and prepared medium at 2C-8C.
- Suggested g/L: 41.006
- Storage Temperature: 2-8 C